WebpCAGGS-mTagBFP2-T2A-iCreERT2 (PV) Plasmid #128171 Purpose Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus) Depositor Mario Capecchi Insert mTagBFP2-T2A-iCreERT2 Use Cre/Lox Tags Expression Mammalian Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pCAGGS-mTagBFP2-T2A-iCreERT2 … Web030197 B6;129S- Pdyn tm1.1(cre/ERT2)Hze /J Pdyn-T2A-CreERT2-D knock-in mice express tamoxifen-inducible Cre recombinase from the endogenous prodynorphin promoter/enhancer elements. When induced, CreERT2 expression/activity is observed mainly in central amygdalar nucleus, paraventricular hypothalamic nucleus, nucleus …
Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and
WebMar 8, 2024 · Published: 08 March 2024 Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background Lech... WebThe Cre recombinase was fused to a modified estrogen receptor (CreERT2) and inserted to the end of the open reading frame of the Tph1 gene at exon 11 with a self-cleaving peptide, T2A (Figure 1Aa and b). The recombinant transcript with T2A (Tph1-T2A-CreERT2) allows the peptide to be cleaved into 2 proteins, TPH1 and CreERT2. the ven. elizabeth adekunle
Dynamic expression of transcription factors T-bet and GATA-3 by ...
WebAug 11, 2024 · Next, variants of T2A-iCre-ER T2 were constructed, with variations at the N-terminus of iCre-ER T2 after the ribosome skip. The sfGFP-iCre-ER T2 was included as a control. The mTagBFP2 was initially cloned in as a transfection marker to normalize for transfection efficiency. WebMar 1, 2024 · The TbetZsGreen-T2A-CreERT2 driver was also linked to a role of age-associated B cells in driving lupus in mice [ 51 ]. Fate mappers have informed our understanding of the development and function of ILCs. WebMar 1, 2024 · Using a TbetZsGreen-T2A-CreERT2 driver, a subset of T follicular helper cells (Tfh) was revealed to express the proinflammatory cytokine IFN-γ in germinal centers … the ven. irene egmalis-maliaman