WebNov 22, 2016 · If you do not use a packing reservoir on top of your column, go directly to (m). The entire bed should reside in the lower column section if using a packing reservoir. Using a pipette or pump, siphon the supernatant from the upper reservoir. Remove the upper reservoir and the coupling ring. WebIf using a packing reservoir, immediately fill the remainder of the column and reservoir with water. Mount the adapter or lid of the packing reservoir and connect the column to a pump. Avoid trapping air bubbles under the adapter or in the inlet tubing. Open the bottom outlet of the column and set the pump to run at the desired flow rate.
About Nova Parks
WebThe Sepharose resins mentioned above are part of the Cytiva BioProcess range of resins. BioProcess chromatography resins are developed and supported for production-scale … WebClose the outlet. 2 Screw the Packing Connector (8) onto the top of the column to be packed (9). 3 Attach the column that serves as reservoir (7). 4 Pour the gel suspension into the reservoir and the column (7). 5 Fit the AK adapter (6) to the reservoir and lower it to the surface of the gel suspension. office 365 on phone
TerraCycle and Cytiva expand first-of-its kind recycling programme
WebSlide the adapter slowly down the column (the outlet of the adapter should be open) until the mark is reached. Lock the adapter in position. Connect the column to the pump and begin equilibration. Reposition the adapter … WebRC packing reservoirs, which are available for all three C column types, are quick and easy to assemble. Curved walls minimize the separation of matrix beads by size during … WebPacking Reservoirs RK 16/26, RK 50 Instruction XK columns are designed for standard liquid chromatography of macromolecules. They are available in the following … mychart hennepin healthcare