Cisplatin binding to holliday junction
WebHolliday junctions (Holliday, 1964) are four-way junctions in DNA formed by the mutual exchange of strand connectivity, and thus connected by the covalent continuity of each strand of the junction ( Orr-Weaver, Szostak, & Rothstein, 1981; Potter & Dressler, 1976; Schwacha & Kleckner, 1995 ). WebFour-way DNA intermediates, called Holliday junctions (HJs), can form during meiotic and mitoticrecombination,andtheirremovaliscrucialforchromosomesegregation.Agroupof ubiquitous and highly specialized structure-selective endonucleases catalyze the cleavage of HJs into two disconnected DNA duplexes in a reaction called HJ resolution.
Cisplatin binding to holliday junction
Did you know?
WebStudy with Quizlet and memorize flashcards containing terms like The zipper-like connection between paired homologs in early prophase is known as a, Crossing-over takes place in bivalents (tetrads) consisting of ______ chromatids, and one crossover involves _______ chromatids., Which type of tetrad contains two recombinant and two parental … WebConformational switching of the synthetic Holliday junction (HJ) labeled with a Cy3-Cy5 fluorophore pair. (b) Representative single molecule fluorescence-time traces from our …
WebThe BCDX2 complex also bound to the synthetic Holliday junction, and its af®nity to the Holliday-junction DNA was signi®cantly higher than that of Rad51B (Fig. 4). WebAug 5, 2011 · Holliday junctions are formed during recombination, double-strand break repair, and fork reversal during replication. Resolving Holliday junctions is a critical process for maintaining genomic stability [ 25, 26 ]. These junctions are resolved by a class of structure-specific nucleases: the junction-resolving enzymes. Figure 1
WebMar 15, 1997 · The results confirm that RuvC protein binds Holliday junctions as a dimer. Influence of DNA sequence on incision Previously it was shown that Holliday junction cleavage by RuvC occurs at specific sequences, with the consensus defined as 5′- A / T TT ↓G / C -3′. Single base changes within this sequence severely reduce the efficiency of … WebMay 15, 2007 · This binding mode is specific for the Holliday junction and does not cause unwinding of the DNA helices. Citing Literature Supporting Information Volume 46, Issue …
WebAug 27, 2024 · Brogden, A.L.; Hopcroft, N.H.; Searcey, M.; Cardin, C.J. Ligand bridging of the DNA Holliday junction: Molecular recognition of a stacked-X four-way junction by a …
WebThe binding of Holliday junction DNA by endo-exonuclease. Purified endo-exonuclease (5 units) was incubated with 32P-labeled junction molecules, linear ss-DNA (146 nucleotides), and linear ds-DNA ... florida zoning ormhWebDNA polymerases fill in 3′ overhangs, whereby the D-loop becomes a Holliday junction. RAD52 functions to anneal the second end of DNA leading to a Holliday junction. BLM migrates the two ends of the Holliday junction in the direction of one another with some filling in by polymerases if necessary. florid cardiogenic shockflorida zoos and animal parksWebApr 3, 2007 · The bifunctional binding of the anticancer drug cisplatin to two adjacent nucleobases in DNA is modeled using density functional theory. Previous experimental studies revealed that cisplatin binding to adjacent guanine and adenine is sensitive to nucleobase sequence. florid counterpointWebFeb 21, 1997 · The E. coli RuvA and RuvB proteins, which are involved in the late stages of recombination and the recombinational repair of damaged DNA, bind to Holliday junctions and promote branch migration. We have used electron microscopy and image analysis to examine RuvA and RuvB bound to model Holliday structures. florid cemento-osseousWebJan 4, 2024 · Organometallic Pillarplexes that bind DNA 4-way Holliday Junctions and Forks bioRxiv. bioRxiv posts many COVID19-related papers. A reminder: they have not … great wolf lodge manteca imagesWebJul 11, 2000 · Actually, both of the RuvA tetramers, the upper and the lower, simultaneously contact each strand of a DNA duplex arm ( 16 ). By contrast, in complex I, the two … great wolf lodge manteca magic quest