Flag-tag mouse monoclonal antibody

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August undefined, 2024

WebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … WebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody

DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to …

WebThis antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Protocols Standard Protocols Click here to view our Standard Protocols Publications All (610) WB … WebOct 4, 2024 · The FLAG tags are attached to the N-terminus or C-terminus of the target proteins. The tagged protein binds to the specific monoclonal antibodies conjugated on the matrix of magnetic beads or agarose gel. The residual impurities are washed off using the reagent washing buffer. how many members are there in everglow

PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody ... - Abcam

WebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized peptide. Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate. β-Actin Rabbit Polyclonal Antibody WebThe MA1-142 antibody (clone L5) has been successfully used as a detection antibody in a direct ELISA with FLAG-tagged protein-coated plates. Similar Epitope as the FLAG tag … WebJun 15, 2012 · The current study reports the successful establishment of hybridoma cells that produce an extremely high-affinity antibody to FLAG, namely 2H8 Ab. 2H8 Ab … how are jewels formed

DYKDDDDK Tag Monoclonal Antibody (FG4R), HRP - Thermo Fisher Scientific

A high-affinity monoclonal antibody against the FLAG tag …

WebMouse monoclonal [F-tag-01] antibody to DDDDK Tag. Applications: ICC, WB: Reactivity: Species independent: Conjugate: Unconjugated: ... Anti-FLAG Tag Antibody [FG4R] (A85282) 10µg - 200µg / £90 – £280 1 Citation. Description: Mouse monoclonal (FG4R) antibody to DYKDDDDK Tag. Applications: WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. how are jigs identifiedWebMouse Antibody Monoclonal. Introduction. A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. … how many members are there in wjsn

"WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. " - Flag-tag mouse monoclonal antibody

Flag-tag mouse monoclonal antibody

DYKDDDDK tag antibody (20543-1-AP) Proteintech - ptglab

WebThe FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. WebDYKDDDDK Tag Monoclonal Antibody ... -Mouse Monoclonal Antibody Acquista DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™ su. Italiano English; Change Country. Ricevi tutte le ultime informazioni Centro di Assistenza e Supporto › Italiano English; Change Country; 0. No offer available; Struttura chimica ...

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WebDYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic … WebAntibodies that detect DYKDDDDK Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Flow Cytometry. Our DYKDDDDK Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Rat, Goat and …

Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M WebWestern blot was performed using HRP-conjugated Anti-DYKDDDDK Mouse Monoclonal Antibody (Product # MA1-91878-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), HA-H3-FLAG, 40 µg (Lane 3), HA-H3-FLAG, 20 µg (Lane 4), HA …

WebJun 15, 2012 · The FLAG sequence (DYKDDDDK) is an artificial sequence widely used to detect, quantify, and purify proteins expressed as FLAG-fusion proteins. Several highly specific monoclonal antibodies for FLAG are commercially available; however, they are not always sensitive enough to detect proteins expressed … http://www.abpbiosciences.com/product/flag-tag/

WebPolyclonal Antibody for studying Flag-Tag. Cited in 708 publications. Validated for WB, IP, F. Available in 2 sizes. ... (G3A1) mAb IgG1 Isotype Control #5415 for mouse monoclonal IgG1 primary antibodies, Mouse …

Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M how are job offers presentedWebKey features and details. Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization. Reacts with: … how are jobs associated with drone technologyWebThe FLAG™ Tag (peptide sequence DYKDDDK) is a short, ... The membrane was probed with DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 488 (Cat. No. MA1-142-A488) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging … how are job grades determinedWebThe Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides. Packaging 0.2, 1, 1 mg Application how many members are there in slipknotWebMonoclonal ANTI-FLAG ® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells. … how are jigsaw puzzles cutWebFlag Tag Monoclonal Antibody Datasheet Product Details Citations Customer Reviews and Q&A Product Details Citations Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2024 how are job offers sentWebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and … how are jigsaw puzzles manufactured